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Open Access Research article

Amblyomma cajennense (Fabricius, 1787) (Acari: Ixodidae), the Cayenne tick: phylogeography and evidence for allopatric speciation

Lorenza Beati1*, Santiago Nava2, Erica J Burkman13, Darci M Barros-Battesti4, Marcelo B Labruna5, Alberto A Guglielmone2, Abraham G Cáceres67, Carmen M Guzmán-Cornejo8, Renato León9, Lance A Durden10 and João LH Faccini11

Author Affiliations

1 Institute for Coastal Plain Sciences and Biology Department, Georgia Southern University, P.O. Box 8056, Statesboro, GA 30460, USA

2 Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Rafaela, CC 22, CP 2300 Rafaela, Santa Fe, Argentina

3 Department of Infectious Diseases, University of Georgia College of Veterinary Medicine, 501 D.W. Brooks Drive Athens, GA 30602, USA

4 Laboratório de Parasitologia, Instituto Butantan, Av. Vital Brasil 1500, 05503-900 São Paulo, SP, Brazil

5 Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, 05508-270, Brazil

6 Departamento Académico de Microbiologia Médica, Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima, Perú

7 Laboratorio de Entomología, Instituto Nacional de Salud, Lima, Perú

8 Laboratorio de Acarología, Departamento de Biología Comparada, Facultad de Ciencias, Universidad Nacional Autónoma de México, Coyoacán 04510, Distrito Federal, México

9 Laboratorio de Entomología Médica y Medicina Tropical (LEMMT), Colegio de Ciencias Biológicas y Ambientales, Universidad San Francisco de Quito, Cumbayá, Quito, Ecuador

10 Biology Department, Georgia Southern University, P.O. Box 8042, Statesboro, GA 30460, USA

11 Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, 23890-000 Seropédica, RJ, Brazil

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BMC Evolutionary Biology 2013, 13:267  doi:10.1186/1471-2148-13-267

Published: 9 December 2013

Abstract

Background

Amblyomma cajennense F. is one of the best known and studied ticks in the New World because of its very wide distribution, its economical importance as pest of domestic ungulates, and its association with a variety of animal and human pathogens. Recent observations, however, have challenged the taxonomic status of this tick and indicated that intraspecific cryptic speciation might be occurring. In the present study, we investigate the evolutionary and demographic history of this tick and examine its genetic structure based on the analyses of three mitochondrial (12SrDNA, d-loop, and COII) and one nuclear (ITS2) genes. Because A. cajennense is characterized by a typical trans-Amazonian distribution, lineage divergence dating is also performed to establish whether genetic diversity can be linked to dated vicariant events which shaped the topology of the Neotropics.

Results

Total evidence analyses of the concatenated mtDNA and nuclear + mtDNA datasets resulted in well-resolved and fully congruent reconstructions of the relationships within A. cajennense. The phylogenetic analyses consistently found A. cajennense to be monophyletic and to be separated into six genetic units defined by mutually exclusive haplotype compositions and habitat associations. Also, genetic divergence values showed that these lineages are as distinct from each other as recognized separate species of the same genus. The six clades are deeply split and node dating indicates that they started diverging in the middle-late Miocene.

Conclusions

Behavioral differences and the results of laboratory cross-breeding experiments had already indicated that A. cajennense might be a complex of distinct taxonomic units. The combined and congruent mitochondrial and nuclear genetic evidence from this study reveals that A. cajennense is an assembly of six distinct species which have evolved separately from each other since at least 13.2 million years ago (Mya) in the earliest and 3.3 Mya in the latest lineages. The temporal and spatial diversification modes of the six lineages overlap the phylogeographical history of other organisms with similar extant trans-Amazonian distributions and are consistent with the present prevailing hypothesis that Neotropical diversity often finds its origins in the Miocene, after the Andean uplift changed the topology and consequently the climate and ecology of the Neotropics.