|Spectral absorbance characteristics for wild type C. nuchalis SWS1 pigments, site-directed mutants, and ancestral pigments|
|Pigment||λmaxa(nm)||Shift fromC. nuchaliswt pigmentb(nm)|
|GBS1 wt||402.97 ± 0.22|
|S90C||363.05 ± 0.05||-40|
|C86S||403.05 ± 0.12||0|
|C86Fc||365.72 ± 1.10||-37|
|C86S/S90C||362.95 ± 0.26||-40|
|Parrot/Passerine Ancestor||402.93 ± 0.28||0|
|Passerine Ancestor||404.28 ± 0.28||0|
a λmax values are given as mean ± standard deviation from at least three different measurements of dark absorbance spectra per expression. b λmax shifts from C. nuchalis wild type (wt) pigment are expressed as negative for blue shifts. c λmax of single mutant C86F calculated from fitting difference spectra of dark and acid denatured species.
van Hazel et al.
van Hazel et al. BMC Evolutionary Biology 2013 13:250 doi:10.1186/1471-2148-13-250