Table 1

Spectral absorbance characteristics for wild type C. nuchalis SWS1 pigments, site-directed mutants, and ancestral pigments
Pigment λmaxa(nm) Shift fromC. nuchaliswt pigmentb(nm)
GBS1 wt 402.97 ± 0.22
S90C 363.05 ± 0.05 -40
C86S 403.05 ± 0.12 0
C86Fc 365.72 ± 1.10 -37
C86S/S90C 362.95 ± 0.26 -40
Parrot/Passerine Ancestor 402.93 ± 0.28 0
Passerine Ancestor 404.28 ± 0.28 0

a λmax values are given as mean ± standard deviation from at least three different measurements of dark absorbance spectra per expression. b λmax shifts from C. nuchalis wild type (wt) pigment are expressed as negative for blue shifts. c λmax of single mutant C86F calculated from fitting difference spectra of dark and acid denatured species.

van Hazel et al.

van Hazel et al. BMC Evolutionary Biology 2013 13:250   doi:10.1186/1471-2148-13-250

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