Additional file 5.

Organisation of the ventral nervous system at the posterior end in the tardigrade Macrobiotus cf. harmsworthi. Serotonin-like immunoreactivity (glow mode) and DNA labelling (light blue); confocal micrographs. Arrowheads point to the somata of serotonin-like immunoreactive neurons. (A) Specimen in ventral view; anterior is up. Note the absence of signal in the interpedal commissure (asterisk). (B–D) Details of the posterior end showing the position of two platelet-shaped, serotonin-like immunoreactive structures (=putative release cites) associated with cloacal nerves (arrows). Abbreviations: cl, cloacal nerve; cn, connectives; ga3–ga4, trunk ganglia 3 to 4. Scale bars: 10 μm (A–D).

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Mayer et al. BMC Evolutionary Biology 2013 13:230   doi:10.1186/1471-2148-13-230