Additional file 3.

Sequence analysis of the MA3 domains of PDCD4 homologs in algae and algal relatives suggests domain duplication in at least some chlorophytes. A phylogenetic tree was generated using the sequences comprising MA3 domains 1–2 and MA3 domains 3–4 of the four MA3 domain PDCD4 homologs of chlorophytes Chlamydomonas reinhardtii and Volvox carteri, and MA3 domains 1–2 and MA3 domain 3 from the partial cDNA of the Chlorella variabilis PDCD4 homolog, MA3 domains 1–2 of the two MA3 domain PDCD4 homologs of prasinophyte Micromonas species and Ostreococcus species, and those of the stramenopiles Ectocarpus siliculosus, Aureococcus anophagefferens, Phaeodactylum tricornutum, Thalassiisira pseudoonana, Phytophthora species, and Albugo laibachii. The phylogenetic tree was constructed using the maximum-likelihood method. Numbers on each branch denote percentages of bootstrap support. Chlorophytes are shaded dark green, species of the Mamiellales are shaded light green, and the stramenopiles are shaded tan.

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Cheng et al. BMC Evolutionary Biology 2013 13:199   doi:10.1186/1471-2148-13-199