Effect of sexual activity post infection on survivorship of males. In this experiment, we infected virgin males (n = 100) with Pe. One hour after infection, 50 males were combined with virgin females while 50 were held in single sex groups. Mortality of the flies in the two treatments was then recorded. Survival of Virgin BRB males held with females post infection (Δ) was not different from that of Virgin males held without females (□) post infection (p = 0.76, Cox proportion regression analysis). None of the sham infected flies died. While we did have two types of sham infected controls (virgin males sham infected and virgin males sham infected and then combined with females), for simplicity, we represent both of them using a single line (―――) in the figure.
Gupta et al. BMC Evolutionary Biology 2013 13:185 doi:10.1186/1471-2148-13-185