Coevolution analyses within GroES and GroEL. The network of coevolving amino acid sites within GroES is shown using the three-letter amino acid code (a) Sites coevolving within GroES were divided into two main structure clusters (b) One cluster includes two amino acid sites (blue spheres), which are involved in the interaction with GroEL. The second cluster includes residues (yellow spheres) mapping to the inter-GroES subunit faces. The network of coevolution in GroEL (c) identifies amino acid sites which are involved in the interaction with GroES and protein substrates (blue spheres in the structure of GroEL: d) sites involved in the inter-subunit GroEL contacts and and substrate folding in the ring cavity (red spheres), residues with a role in ATP hydrolysis (green sphere) and those mapping to the inter-ring interfaces (black spheres).
Ruiz-González and Fares BMC Evolutionary Biology 2013 13:156 doi:10.1186/1471-2148-13-156