Open Access Highly Accessed Research article

Persistence across Pleistocene ice ages in Mediterranean and extra-Mediterranean refugia: phylogeographic insights from the common wall lizard

Daniele Salvi*, D James Harris, Antigoni Kaliontzopoulou, Miguel A Carretero and Catarina Pinho

Author Affiliations

CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto; Campus Agrário de Vairão, 4485-661, Vairão, Portugal

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BMC Evolutionary Biology 2013, 13:147  doi:10.1186/1471-2148-13-147

Published: 11 July 2013

Additional files

Additional file 1:

Details on Sequences from GenBank used in the study. Accession numbers, localities, and references for the 103 cytb sequences obtained from GenBank used in the phylogenetic analyses.

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Additional file 2:

Primers used for amplification and sequencing. Primers sequences and reference for each gene are reported.

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Additional file 3:

Times to the most recent common ancestor estimates. Times to the most recent common ancestor of supported mitochondrial DNA clades within P. muralis (in million years) estimated in BEAST. Values in parenthesis refer to the lower and higher limits of the 95% highest posterior density interval.

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Additional file 4:

SAMOVA (Spatial Analysis of Molecular Variance) design and results. A: Geographic location of mitochondrial clades estimated as the centroid between member localities (see also Figure 1). B: Localities and sequences included in each mitochondrial clade as defined by previous phylogenetic analyses. C: SAMOVA results showing relative fixation indices FCT and FSC (P < 0.001) for pre-defined value of K from 2 to 10 (after K=10 structures are not informative as one population at a time is removed from the groups structure).

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Additional file 5:

Statistical comparison between the hypotheses of southern vs. in situ origin of northern localities.

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