Open Access Research article

Evolution of the myosin heavy chain gene MYH14 and its intronic microRNA miR-499: muscle-specific miR-499 expression persists in the absence of the ancestral host gene

Sharmin Siddique Bhuiyan1, Shigeharu Kinoshita1*, Chaninya Wongwarangkana1, Md Asaduzzaman1, Shuichi Asakawa1 and Shugo Watabe12

Author Affiliations

1 Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo 113-8657, Japan

2 School of Marine Bioscience, Kitasato University, Minami, Sagamihara, Kanagawa 252-0373, Japan

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BMC Evolutionary Biology 2013, 13:142  doi:10.1186/1471-2148-13-142

Published: 6 July 2013

Additional files

Additional file 1: Figure S1:

MYH14 and mIR-499 phylogenetic analysis. MYH14 (A) and miR-499 (B) maximum-likelihood (ML) trees. Bootstrap values from 1000 replicates analysis are given at the nodes as percentage values.

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Additional file 2: Figure S2:

Sequence comparison of the intron containing miR-499 among torafugu, zebrafish, and medaka. Shaded sequences are highly conserved regions among the three fish species. Mature miR-499 sequences are boxed. Bold letters indicate 5′ and 3′ intron splice sites. Numbers on the right indicate the positions of the MYH14 (torafugu and zebrafish) start codon and mature miR-499 (medaka) 5′-end. Nucleotide sequences were aligned by CLUSTALW.

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Additional file 3: Figure S3:

Intronic conserved regions in MYH14 among torafugu, zebrafish, and medaka. The red box shows highly conserved regions among the three fish species. Bold letters indicate 5′ and 3′ splice intron sites. Numbers on the right indicate the positions of the MYH14 (torafugu and zebrafish) start codon and mature miR-499 (medaka) 5′-end. Nucleotide sequences were aligned by CLUSTALW.

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Additional file 4: Figure S4:

5′-flanking conserved regions in MYH14 among torafugu, zebrafish, and medaka. The red and gray boxes show highly conserved regions between torafugu and medaka, and among the three fish species, respectively. Bold letters indicate 5′ and 3′ splice intron sites. Numbers on the right indicate the positions of the MYH14 (torafugu and zebrafish) start codon and mature miR-499 (medaka) 5′-end. Nucleotide sequences were aligned by CLUSTALW.

Format: ZIP Size: 170KB Download file

Open Data