K2P-distance analyses of tRNA-Met genes and the tRNA-Met pseudogenes found in Diaphini and Notolychnus. A. Analysis done with randomly selected myctophid tRNA-Met genes using a neoscopelid outgroup. B. Analysis done with only diaphinid taxa and Notolychnus using a neoscopelid outgroup. Note the better phylogenetic structure in the pseudogenes compared to their counterpart tRNA-Met genes. C. Mitogenomic cladogram showing the IMQMψ – region in Diaphini. The truncated branch of Notolychnus signifies a phylogenetic position not corroborated from DNA sequences or tRNA-Met/tRNA-Met pseudogene distance analyses. Shaded areas represent INC-regions with number of base pairs noted for each taxon. Note the “gradual” removal of the INC-regions in diaphinid taxa except the tRNA-Met pseudogene (dark shaded). Secondary folding structures are presented for Notolychnus tRNA-Met1 and Met2 and all diaphinid tRNA-Met pseudogenes.
Poulsen et al. BMC Evolutionary Biology 2013 13:111 doi:10.1186/1471-2148-13-111