Table 5

Competition among different site-directed mutants of bacteriophage Qβ in the presence of AZC
Competitor viruses Viruses present in the final population1
Transfer number 5 Transfer number 20
wt + QβA1746U A1746U ND2
wt + QβA2982G A2982G ND
wt + QβU3582C U3582C ND
A1746U + QβA2982G A1746U ND
A1746U + QβU3582C A1746U ND
A2982G + QβU3582C A2982G + A2982G +
U3582C U3582C
A1746U + QβA1746U + A2982G A1746U + A2982G ND
A1746U + QβA1746U + U3582C A1746U + U3582C ND
A1746U + A2982G + QβA1746U + U3582C A1746U + A2982G + A1746U + A2982G + QβA1746U + U3582C
A1746U + U3582C
A1746U + QβA1746U + A2982G + QβA1746U + U3582C (QβA1746U)3 + (QβA1746U)3 +
A1746U + A2982G + A1746U + A2982G +QβA1746U + U3582C
A1746U + U3582C

1Competitions were carried out as described in Methods. The viruses present in the populations obtained at the transfer indicated were identified through the determination of the consensus sequences at the nucleotide positions that distinguish the competitor viruses.

2ND means not determined.

3In this case, the determination of the consensus sequence do not allow distinguishing whether QβA1746U is present in the populations analyzed or not. We show this virus in brackets to indicate this uncertainty.

Cabanillas et al.

Cabanillas et al. BMC Evolutionary Biology 2013 13:11   doi:10.1186/1471-2148-13-11

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