Table 4

Relative fitness of bacteriophage Qβ site-directed mutants
Site-directed mutant Relative fitness1 Relative fitness1 Dominant virus in competition with Qβwt2
+AZC -AZC +AZC -AZC
A1746U 0.71 ± 0.02* 0.58 ± 0.03* A1746U wt
A2982G 1.24 ± 0.08* 0.89 ± 0.05* A2982G wt
U3582C 1.31 ± 0.03* 0.89 ± 0.06* U3582C wt
C3413G3 0.95 ± 0.04* 0.92 ± 0.07* C3413G +wt (transfer 15) C3413G +wt (transfer15)
G3945A3 1.1 ± 0.06* 1.02 ± 0.05 G3945A (transfer15) G3945A (transfer15)
U3989C 0.79 ± 0.04* 0.88 ± 0.05* wt wt

1Relative fitness was evaluated with respect to the virus Qβwt either in the absence or the presence of AZC (60 μg/ml). The absolute fitness value estimated for Qβwt was 15.42 ± 0.50 in the absence of AZC and 9.68 ± 0.22 in its presence. The asterisk indicates that the difference between the fitness values of the site-directed mutant and the virus Qβwt was significant (p value < 0.05, Student’s t test for the difference of means).

2Competition between the site-directed mutants indicated and the virus Qβwt was carried out as described in Methods during 5 transfers either in the absence or the presence of AZC (60 μg/ml).

3Since no virus became dominant at transfer number 5, competitions carried out with viruses QβC3413G and QβG3945A were extended until transfer number 15, yielding the results shown in the table.

Cabanillas et al.

Cabanillas et al. BMC Evolutionary Biology 2013 13:11   doi:10.1186/1471-2148-13-11

Open Data