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Open Access Highly Accessed Research article

Evolutionary genomics of mycovirus-related dsRNA viruses reveals cross-family horizontal gene transfer and evolution of diverse viral lineages

Huiquan Liu125, Yanping Fu2, Jiatao Xie2, Jiasen Cheng2, Said A Ghabrial3, Guoqing Li12, Youliang Peng4, Xianhong Yi2 and Daohong Jiang12*

Author affiliations

1 State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei Province, People’s Republic of China

2 The Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei Province, People’s Republic of China

3 Department of Plant Pathology, University of Kentucky, 201 F Plant Science Building, 1405 Veterans Drive, Lexington, KY, 40546-0312, USA

4 State Key Laboratories for Agrobiotechnology, China Agricultural University, Yuanming-Yuan West Road No. 2, Haidian District, 100193, Beijing, People’s Republic of China

5 Purdue-NWAFU Joint Research Center, Northwest A&F University, Yangling, 712100, Shaanxi Province, People’s Republic of China

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Citation and License

BMC Evolutionary Biology 2012, 12:91  doi:10.1186/1471-2148-12-91

Published: 20 June 2012

Abstract

Background

Double-stranded (ds) RNA fungal viruses are typically isometric single-shelled particles that are classified into three families, Totiviridae, Partitiviridae and Chrysoviridae, the members of which possess monopartite, bipartite and quadripartite genomes, respectively. Recent findings revealed that mycovirus-related dsRNA viruses are more diverse than previously recognized. Although an increasing number of viral complete genomic sequences have become available, the evolution of these diverse dsRNA viruses remains to be clarified. This is particularly so since there is little evidence for horizontal gene transfer (HGT) among dsRNA viruses.

Results

In this study, we report the molecular properties of two novel dsRNA mycoviruses that were isolated from a field strain of Sclerotinia sclerotiorum, Sunf-M: one is a large monopartite virus representing a distinct evolutionary lineage of dsRNA viruses; the other is a new member of the family Partitiviridae. Comprehensive phylogenetic analysis and genome comparison revealed that there are at least ten monopartite, three bipartite, one tripartite and three quadripartite lineages in the known dsRNA mycoviruses and that the multipartite lineages have possibly evolved from different monopartite dsRNA viruses. Moreover, we found that homologs of the S7 Domain, characteristic of members of the genus phytoreovirus in family Reoviridae are widely distributed in diverse dsRNA viral lineages, including chrysoviruses, endornaviruses and some unclassified dsRNA mycoviruses. We further provided evidence that multiple HGT events may have occurred among these dsRNA viruses from different families.

Conclusions

Our study provides an insight into the phylogeny and evolution of mycovirus-related dsRNA viruses and reveals that the occurrence of HGT between different virus species and the development of multipartite genomes during evolution are important macroevolutionary mechanisms in dsRNA viruses.