Relationship between levels of co-infection and genetic diversity of WNV. Bars depict normalized Shannon entropy (Sn) on both the nucleotide (nt) and amino acid (aa) levels and circles depict relative differences in mean hamming distance for populations following a single passage of WNV CP40 at indicated MOIs. All measures of genetic diversity are for nt 1311-3248 and 17-22 clones were sequenced and analyzed for each measure. A significant positive correlation between co-infection (MOI) and Sn for both nt and aa was measured (linear regression analyses, r2 = 0.99, p < 0.05).
Ciota et al. BMC Evolutionary Biology 2012 12:58 doi:10.1186/1471-2148-12-58