Table 2

Stresses performed on female-sterile strains S1-B12 and S3-A10 and the corresponding wild-type strains S1 and S3

Proportion of female-fertile replicates before treatment

Proportion of female-fertile replicates after treatments



Stress

S1

S3

S1-B12

S1-A10

S1

S3

S1-B12

S3-A10



-80°C

(1 h)

5/5

5/5

0/5

0/5

5/5

5/5

0/5

0/5

-80°C

(24 h)

5/5

5/5

0/5

0/5

5/5

5/5

0/5

0/5

Mycelium

mashing

5/5

5/5

0/5

0/5

5/5

5/5

0/5

0/5

single-spore

isolation

1/1

1/1

0/1

0/1

12/12

12/12

0/12

0/10

growth on plant

1/1

1/1

0/1

0/1

7/10

10/10

0/8

0/10


The treatments were: one hour at -80°C, 24 hours at -80°C, mycelium mashing by ultrasound, single-spore isolation and growth on plants. The ratios (number of replicates in which the strain was female-fertile)/(number of replicates in which the strain was female-sterile) are given before and after the various treatments

Saleh et al. BMC Evolutionary Biology 2012 12:42   doi:10.1186/1471-2148-12-42

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