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Open Access Highly Accessed Research article

Winding up the molecular clock in the genus Carabus (Coleoptera: Carabidae): assessment of methodological decisions on rate and node age estimation

Carmelo Andújar1*, José Serrano1 and Jesús Gómez-Zurita2

Author Affiliations

1 Departamento de Zoología y Antropología Física. Facultad de Veterinaria, Universidad de Murcia, 30071 Murcia, Spain

2 Institut de Biologia Evolutiva (CSIC-UPF), Pg. Marítim de la Barceloneta 37, 08003 Barcelona, Spain

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BMC Evolutionary Biology 2012, 12:40  doi:10.1186/1471-2148-12-40

Published: 28 March 2012

Additional files

Additional file 1:

Additional file. One PDF file including: A) Supporting Figures and Legends. Figure S1 to S12. Phylogenetic trees of the genus Carabus obtained with MrBayes, BEAST (outgroup dataset) and BEAST (ingroup dataset) under the selected parameters. Bars represent 95% confident intervals for the node ages in Ma. Numbers inside nodes represent posterior probabilities. S1. cox1-A; S2. cox1-B; S3. cob; S4. nd5; S5. rrnL; S6. LSU-A; S7. LSU-B; S8. ITS2; S9. HUWE1. S10. MIT; S11. NUC; S12. MIT-NUC. B) Supporting Tables. Table S1 Primers used in the molecular clock calibration study of the genus Carabus. Table S2 Data about nd5 sequences and specimens of the genus Carabus and related taxa employed to conduct initial calibration analyses. Table S3 Calculations for the objective selection of alignments. Table S4. Marginal likelihood values in BEAST analyses for individual and combined gene fragments as estimated in Tracer v1.5. Table S5 Mean rates of molecular evolution and 95% HPD intervals in calibration analyses on the genus Carabus. Table S6 Mean ages and 95% HPD intervals in calibration analyses on the genus Carabus. C) Supplementary Text D) Supplementary References.

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