Additional file 1.

Figure S1. Transcription factor expression in pre-osteoblasts of gar. A, Trichrome-stained coronal section. B, C, in situ hybridization on coronal sections. Trichrome staining of 7 dpf gar (A) shows mesenchymal cells lateral to the condensation of Meckel's cartilage. These pre-osteoblasts do not express sox9 (B), but express high levels of runx2 (C). Scale bars: A-C = 50 μm. Abbreviations: dpf = days post-fertilization; Mk = Meckel's; po = pre-osteoblasts; Tri = Trichrome.

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Eames et al. BMC Evolutionary Biology 2012 12:27   doi:10.1186/1471-2148-12-27