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Open Access Highly Accessed Research article

Evolution of the osteoblast: skeletogenesis in gar and zebrafish

B Frank Eames12*, Angel Amores1, Yi-Lin Yan1 and John H Postlethwait1

Author Affiliations

1 Institute of Neuroscience, University of Oregon, Eugene, OR 97403-1254, USA

2 Department Anatomy and Cell Biology, University of Saskatchewan, 107 Wiggins Road, Saskatoon, SK S7N 5E5, Canada

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BMC Evolutionary Biology 2012, 12:27  doi:10.1186/1471-2148-12-27

Published: 5 March 2012

Additional files

Additional file 1:

Figure S1. Transcription factor expression in pre-osteoblasts of gar. A, Trichrome-stained coronal section. B, C, in situ hybridization on coronal sections. Trichrome staining of 7 dpf gar (A) shows mesenchymal cells lateral to the condensation of Meckel's cartilage. These pre-osteoblasts do not express sox9 (B), but express high levels of runx2 (C). Scale bars: A-C = 50 μm. Abbreviations: dpf = days post-fertilization; Mk = Meckel's; po = pre-osteoblasts; Tri = Trichrome.

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