Additional file 2.

Figure S1. P element insertion mutation in the Hsp83 proximal promoter reduces Hsp83 gene expression. (A) Hsp83 gene promoter-luciferase reporter constructs (see also Materials and methods). Promoters of the Hsp83 gene (both as wild-type and with P-element insertion) from the Okayama population were amplified, digested with Kpn1 and Bgl2, and ligated into the promoter region of the luciferase gene in the pGL3-basic vector as described in Materials and Methods. HSC, Heat-shock consensus element; TATA, TATA box. Constructs are not drawn to scale. (B) Hsp83 gene promoter activity, as measured by a luciferase assay (see also Materials and methods). Drosophila S2R+ cells and Kc cells were transiently transfected in separate experiments with luciferase constructs that contained the wild-type Hsp83 promoter, and the same promoter with an inserted P element. Transfectants were subject to control (i.e., non-heat-shock) or 37°C heat-shock treatments, as described in Materials and methods. The vertical axis shows the firefly luciferase signal, expressed as the ratio of firefly to Renilla luciferase luminescence measured from 4 replicate cell lines for each treatment.

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Chen and Wagner BMC Evolutionary Biology 2012 12:25   doi:10.1186/1471-2148-12-25