Table 4

Results of nested ANOVAs for longevity
A) Longevity DF MS F P
Selection Regime 1,6 234.0 0.6 0.4616
Replicate Line [Sel. & Inbr.] 6,3744 404.9 2.8 0.0109
Inbreeding Level 2,6 146.3 0.4 0.6967
Rearing Temperature 1,3744 264262.0 1806.6 <0.0001
Sex 1,3744 20.7 0.14 0.7070
Selection Reg. * Inbreeding Level 2,6 2020.7 5.3 0.0440
Selection Reg. * Rearing Temp. 1,3744 93.4 0.6 0.4242
Selection Reg. * Sex 1,3744 52.2 0.4 0.5505
Inbreeding Level * Rearing Temp. 2,3744 440.1 3.0 0.0495
Inbreeding Level * Sex 2,3744 153.7 1.0 0.3498
Rearing Temp. * Sex 1,3744 5324.0 36.4 <0.0001
Sel. Reg. * Inbreed. * Rear. Temp. 2,3744 472.0 3.2 0.0398
Sel. Reg. * Inbreed. * Sex 2,3744 940.0 6.4 0.0016
Sel. Reg. * Rearing Temp. * Sex 1,3744 1.5 <0.1 0.9202
Inbreed. * Rear. Temp. * Sex 2,3744 178.4 1.2 0.2954
Four-way Interaction 2,3744 932.1 6.4 0.0017
Error 3744 146.3
B) Animals reared at 20°C DF MS F P
Selection Regime 1,6 287.5 0.3 0.6267
Inbreeding Level 2,6 174.0 0.2 0.8570
Replicate Line [Sel. Reg. & Inbr.] 6,1312 1106.3 4.8 <0.0001
Adult Temperature 1,1312 34430.6 148.6 <0.0001
Sex 1,1312 2568.9 11.1 0.0009
Selection Reg. * Inbreeding Level 2.6 1712.1 1.6 0.2864
Selection Reg. * Adult Temp. 1,1312 1726.0 7.5 0.0064
Selection Reg. * Sex 1,1312 7.3 <0.1 0.8589
Inbreeding Level * Adult Temp. 2,1312 3034.3 13.5 <0.0001
Inbreeding Level * Sex 2,1312 131.9 0.6 0.5659
Adult Temperature * Sex 1,1312 2762.2 12.6 0.0004
Sel. Reg. * Inbreed. * Sex 2,1312 1395.7 6.0 0.0025
Error 1312 231.6
C) Animals reared at 27°C DF MS F P
Selection Regime 1,6 38.0 0.1 0.7603
Inbreeding Level 2,6 142.8 0.4 0.6974
Replicate Line [Sel. Reg. & Inbr.] 6,2394 37. 7.0 <0.0001
Adult Temperature 1,2394 1177.7 21.8 <0.0001
Adult Feeding Regime 1,2394 52827.7 977.2 <0.0001
Sex 1,2394 3684.1 68.1 <0.0001
Selection Reg. * Inbreeding Level 2,6 475.6 1.3 0.3451
Selection Reg. * Adult Temp. 1,2394 104.6 1.9 0.1644
Selection Reg. * Adult Feed. 1,2394 49.3 0.9 0.3399
Selection Reg. * Sex 1,2394 15.4 0.3 0.5936
Inbreeding Level * Adult Temp. 2,2394 37.1 0.7 0.5033
Inbreeding Level * Adult Feed. 2,2394 112.8 2.1 0.1244
Inbreeding Level * Sex 2,2394 137.9 2.6 0.0782
Adult Temp. * Adult Feed. 1,2394 451.8 8.4 0.0039
Adult Temp. * Sex 1,2394 114.8 2.1 0.1451
Adult Feed. * Sex 1,2394 1481.2 27.4 <0.0001
Sel. Reg. * Inbreed. * Adult Temp. 2,2394 17.4 0.3 0.7248
Sel. Reg. * Inbreed. *Adult Feed. 2,2394 532.5 9.9 <0.0001
Sel. Reg. * Inbreed. * Sex 22394 0.7 <0.1 0.9879
Sel. Reg. * Adult Temp. * Sex 2,2394 1.7 <0.1 0.8610
Sel. Reg. * Adult Temp. * AdFeed. 1,2394 128.8 2.4 0.1229
Sel. Reg. * Adult Feed. * Sex 1,2394 0.7 <0.1 0.9110
Inbreed. * Adult Temp. * AdFeed. 2,2394 14.4 0.3 0.7659
Inbreed. * Adult Temp. * Sex 2,2394 161.9 3.0 0.0502
Inbreed. * Adult Feed. * Sex 2,2394 164.2 3.0 0.0482
Adult Temp. * Adult Feed. * Sex 1,2394 87.1 1.6 0.2044
Inbreed. * AdT * AdF * Sex 2,2394 222.8 4.1 0.0163
Error 2394 54.0

Results for the effects of selection regime (selection vs. control), inbreeding level (C, I1, I2), replicate line, replicate cage, rearing temperature (20 vs. 27°C), and sex on longevity in Bicyclus anynana (A). In Tables B (animals reared at 20°C) and C (animals reared at 27°C) data were analyzed separately for each rearing temperature, as different feeding treatments were employed in animals reared at 27°C only, and as different adult temperatures were employed in animals reared at 27°C only temporarily. Consequently, the analysis in A was restricted to the animals fed ad libitum and kept as adults at 27°C, to mainly explore effects of rearing temperature. The factor rearing temperature was replaced by the factor adult temperature in analyses B and C, and the factor adult feeding regime (control vs. starvation) was added to Table C. For details see Material and Methods. Replicate line (random factor) was nested within selection regime and inbreeding level throughout. Minimum adequate models were constructed by sequentially removing non-significant interaction terms. Significant P-values are given in bold.

Franke et al.

Franke et al. BMC Evolutionary Biology 2012 12:235   doi:10.1186/1471-2148-12-235

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