Table 3

Results of nested ANOVAs for chill-coma recovery time
A) Chill-coma recovery time DF MS F P
Selection Regime 1,6 24368.4 22.0 0.0032
Inbreeding Level 2,6 1183.4 1.1 0.4005
Replicate Line [Sel. & Inbr.] 6,4738 1204.3 7.7 <0.0001
Rearing Temperature 1,4738 127708.0 815.0 <0.0001
Adult Temperature 1,4738 115643.0 738.0 <0.0001
Sex 1,4738 <0.1 <0.1 0.9926
Selection Reg. * Inbreeding Level 2,6 192.6 0.2 0.8448
Selection Reg.* Rearing Temp. 1,4738 480.4 3.1 0.0800
Selection Reg. * Adult Temp. 1,4738 1327.2 8.5 0.0036
Selection Reg. * Sex 1,4738 21.2 0.1 0.7131
Inbreeding Level * Rearing Temp. 2,4738 797.1 5.1 0.0062
Inbreeding Level * Adult Temp. 2,4738 692.4 4.4 0.0121
Inbreeding Level * Sex 2,4738 254.0 1.6 0.1979
Rearing Temp. * Adult Temp. 1,4738 1.2 <0.1 0.9298
Rearing Temp. * Sex 1,4738 1.8 <0.1 0.9140
Adult Temp. * Sex 1,4738 1384.2 8.8 0.0030
Sel. Reg. * Inbreed. * Rear. Temp. 2,4738 910.95 5.8 0.0030
Sel. Reg. * Inbreed. * Adult Temp. 2,4738 527.1 3.4 0.0347
Inbreed. * Rear. Temp. * Adult Temp. 2,4738 504.9 3.2 0.0400
Rear. Temp. * Adult Temp. * Sex 1,4738 1121.7 7.2 0.0075
Error 4738 156.7
B) Animals reared at 20°C DF MS F P
Selection Regime 1,6 6782.9 18.8 0.0049
Inbreeding Level 2,6 213.8 0.6 0.5829
Replicate Line [Sel. & Inbr.] 6,1668 362.6 4.1 0.0004
Adult Temperature 1,1668 44483.1 501.1 <0.0001
Sex 1,1668 1.3 <0.1 0.9046
Selection Reg. * Adult Temp. 1,1668 1458.2 16.4 <0.0001
Error 1668 88.8
C) Animals reared at 27°C DF MS F P
Selection Regime 1,6 23033.8 15.3 0.0079
Inbreeding Level 2,6 2340.1 1.5 0.2859
Replicate Line [Sel. & Inbreed.] 6,3059 1506.7 7.9 <0.0001
Adult Temperature 1,3059 81814.6 427.7 <0.0001
Adult Feeding Regime 1,3059 5.6 <0.1 0.8644
Sex 1,3059 0.4 <0.1 0.9620
Selection Reg. * Inbreeding Level 2,6 959.2 0.6 0.5611
Selection Reg. * Adult Temp. 1,3059 321.2 1.7 0.1952
Selection Reg. * Adult Feed. 1,3059 66.0 0.3 0.5570
Selection Reg. * Sex 1,3059 38.0 0.2 0.6557
Inbreeding Level * Adult Temp. 2,305 1643.0 8.6 0.0002
Inbreeding Level * Adult Feed. 2,3059 211.2 1.1 0.3317
Inbreeding Level * Sex 2,3059 145.2 0.8 0.4682
Adult Temp. * Adult Feed. 1,3059 231.8 1.2 0.2711
Adult Temp. * Sex 1,3059 3459.7 18.1 <0.0001
Adult Feed. * Sex 1,3059 174.4 0.9 0.3397
Sel. Reg. * Inbreed. * Adult Temp. 2,3059 961.3 5.0 0.0066
Inbreed. * Adult Feed. * Sex 2,3059 1243.4 6.5 0.0015
Adult Temp. * Adult Feed. * Sex 1,3059 1362.7 7.1 0.0076
Error 3059 191.3

Results for the effects of selection regime (selection vs. control), inbreeding level (C, I1, I2), replicate line, rearing temperature (20 vs. 27°C), adult temperature (20 vs. 27°C), and sex on chill-coma recovery time in Bicyclus anynana (A). In Tables B (animals reared at 20°C) and C (animals reared at 27°C) data were analyzed separately for each rearing temperature, as different feeding treatments were employed in animals reared at 27°C only. Consequently, the factor rearing temperature was dropped from both analyses, and the factor adult feeding regime (control vs. starvation) was added to Table C. For details see Material and Methods. Replicate line (random factor) was nested within selection regime and inbreeding level throughout. Minimum adequate models were constructed by sequentially removing non-significant interaction terms. Significant P-values are given in bold.

Franke et al.

Franke et al. BMC Evolutionary Biology 2012 12:235   doi:10.1186/1471-2148-12-235

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