Table 2

Results of nested ANOVAs for larval time (A), pupal time (B), larval growth rate (C) and pupal mass (D)
A) Larval time DF MS F P
Selection Regime 1,6 205.6 1.0 0.3453
Inbreeding Level 2,6 87.2 0.4 0.6614
Replicate Line [Sel. Reg. & Inbr.] 6,110 216.0 9.4 <0.0001
Repl. Cage [Sel., Inbr. & Repl.] 108,4640 23.5 3.8 <0.0001
Rearing Temperature 1,4640 565906.0 90265.2 <0.0001
Sex 1,4640 5780.5 922.0 <0.0001
Selection Reg. * Inbreeding Level 2,6 236.5 1.2 0.3635
Selection Reg. * Rearing Temp. 1,4640 107.4 17.1 <0.0001
Selection Reg. * Sex 1,4640 11.2 1.8 0.1819
Inbreeding Level * Rearing Temp. 2,4640 139.2 22.2 <0.0001
Inbreeding Level * Sex 2,4640 14.4 2.3 0.1007
Rearing Temp. * Sex 1,4640 219.6 35.0 <0.0001
Sel. Reg. * Inbreed. * Rear. Temp. 2,4640 49.9 8.0 0.0004
Sel. Reg. * Rearing Temp. * Sex 1,4640 25.0 25.0 0.0457
Error 4640 6.3
B) Pupal time DF MS F P
Selection Regime 1,6 1.4 0.1 0.7627
Inbreeding Level 2,6 9.2 0.6 0.5567
Replicate Line [Sel. Reg. & Inbr.] 6,111 15.6 11.7 <0.0001
Repl. Cage [Sel., Inbr. & Repl.] 108,4639 1.4 2.5 <0.0001
Rearing Temperature 1,4639 24740.3 45323.1 <0.0001
Sex 1,4639 376.4 689.6 <0.0001
Selection Reg. * Inbreeding Level 2,6 1.7 0.1 0.8888
Selection Reg. * Rearing Temp. 1,4639 0.2 0.4 0.5196
Selection Reg. * Sex 1,4639 0.8 8.8 0.3708
Inbreeding Level * Rearing Temp. 2,4639 12.8 23.4 <0.0001
Inbreeding Level * Sex 2,4639 3.0 5.5 0.0041
Rearing Temp. * Sex 1,4639 13.6 24.9 <0.0001
Sel. Reg. * Inbreed. * Rear. Temp. 2,3941 3.5 6.3 0.0018
Inbreed. * Rear. Temp. * Sex 2,4639 2.2 4.1 0.0173
Error 4639 0.5
C) Growth rate DF MS F P
Selection Regime 1,6 5.7 0.1 0.7710
Inbreeding Level 2,6 40.9 0.7 0.5514
Replicate Line [Sel. Reg. & Inbr.] 6,109 68.1 8.2 <0.0001
Repl. Cage [Sel., Inbr. & Repl.] 108,4641 8.6 4.3 <0.0001
Rearing Temperature 1,4641 79417.9 40343.3 <0.0001
Sex 1,4641 154.2 78.3 <0.0001
Selection Reg. * Inbreeding Level 2,6 43.2 0.7 0.5353
Selection Reg. * Rearing Temp. 1,4641 2.1 1.1 0.3012
Selection Reg. * Sex 1,4641 0.4 0.2 0.6716
Inbreeding Level * Rearing Temp. 2,4641 33.0 16.8 <0.0001
Inbreeding Level * Sex 2,4641 0.6 0.3 0.7479
Rearing Temp. * Sex 1,4641 31.3 15.9 <0.0001
Sel. Reg. * Inbreed. * Rear. Temp. 2,4641 8.8 4.5 0.0115
Error 4641 2.0
D) Pupal mass DF MS F P
Selection Regime 1,6 2366.2 0.6 0.4880
Inbreeding Level 2,6 38734.6 8.9 0.0156
Replicate Line [Sel. Reg. & Inbr.] 6,110 4746.8 3.2 0.0064
Repl. Cage [Sel., Inbr. & Repl.] 108,4641 1527.4 3.8 <0.0001
Rearing Temperature 1,4641 156622.0 389.2 <0.0001
Sex 1,4641 1661991.0 4143.6 <0.0001
Selection Reg. * Inbreeding Level 2,6 231.4 0.1 0.9487
Selection Reg. * Rearing Temp. 1,4641 949.7 2.4 0.1239
Selection Reg. * Sex 1,4641 251.4 0.6 0.4286
Inbreeding Level * Rearing Temp. 2,4641 1285.1 3.2 0.0407
Inbreeding Level * Sex 2,4641 1253.2 3.1 0.0441
Rearing Temp. * Sex 1,4641 295.4 0.7 0.3908
Sel. Reg. * Inbreed. * Rear. Temp. 2,4641 2182.8 5.4 0.0044
Error 4641 400.5

Results for the effects of selection regime (selection vs. control), inbreeding level (C, I1, I2), replicate line, replicate cage, rearing temperature (20 vs. 27°C), and sex on larval time (A), pupal time (B), larval growth rate (C), and pupal mass (D) in Bicyclus anynana. Replicate line was nested within selection regime and inbreeding level, and replicate cage was nested within replicate line, inbreeding level and selection regime. Both latter factors were included as random effects, while all others were considered fixed effects. Minimum adequate models were constructed by sequentially removing non-significant interaction terms. Significant P-values are given in bold.

Franke et al.

Franke et al. BMC Evolutionary Biology 2012 12:235   doi:10.1186/1471-2148-12-235

Open Data