Table 2

Primers and Taqman probes used in this study
Gene Primers Taqman probe GenBank accession ID
UV opsin Forward primer: 5′-GCAAGCGAAGAAAATGAACGTAGAA-3′ 5′-CTGCCGCGTTTTGAT-3′ AF484248.1
Reverse Primer: 5′-CTATCCTGATTTCCGCTGACTCT-3′
blue opsin Forward primer: 5′-CGCGAGTGCAAGCATCTC-3′ 5′-TTGCCGTTCACCTTCC-3′ AY918894.1
Reverse primer: 5′-CACGAATTTTCCCCAGATCCTGAA-3′
LW opsin Forward primer: 5′-CGCCTGTGGAACCGATTACTT-3′ 5′-TTGCCACGACTTGTCG-3′ AY918895.2
Reverse primer: 5′-AGCAGAAGATCGAGTAGAACAGGAT-3′

These sequences were used to quantitatively amplify B. anynana UV, blue, and LW opsin cDNAs. Primers and probes for eukaryotic 18S rRNA are proprietary (Applied Biosystems).

Everett et al.

Everett et al. BMC Evolutionary Biology 2012 12:232   doi:10.1186/1471-2148-12-232

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