Additional file 2.

The comparison of nucleotides at the polymorphic sites between uncloned and cloned PCR products. (A) An example that an individual is identified as a heterozygote. The upper figure is the alignment of nucleotide sequences of uncloned and cloned PCR products. The lower figure is the result of direct-sequencing for uncloned PCR product. (B) An example that an individual is not identified as a heterozygote. Such an individual is excluded from analyses of the present study.

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Yasukochi et al. BMC Evolutionary Biology 2012 12:230   doi:10.1186/1471-2148-12-230