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Open Access Highly Accessed Research article

Molecular evolution and phylogenetics of rodent malaria parasites

Ricardo S Ramiro1*, Sarah E Reece123 and Darren J Obbard13

Author Affiliations

1 Institute of Evolutionary Biology, University of Edinburgh, Edinburgh, United Kingdom

2 Institute of Immunology and Infection Research, University of Edinburgh, Edinburgh, United Kingdom

3 Centre for Immunity, Infection and Evolution, University of Edinburgh, Edinburgh, United Kingdom

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BMC Evolutionary Biology 2012, 12:219  doi:10.1186/1471-2148-12-219

Published: 14 November 2012

Abstract

Background

Over the last 6 decades, rodent Plasmodium species have become key model systems for understanding the basic biology of malaria parasites. Cell and molecular parasitology have made much progress in identifying genes underpinning interactions between malaria parasites, hosts, and vectors. However, little attention has been paid to the evolutionary genetics of parasites, which provides context for identifying potential therapeutic targets and for understanding the selective forces shaping parasites in natural populations. Additionally, understanding the relationships between species, subspecies, and strains, is necessary to maximize the utility of rodent malaria parasites as medically important infectious disease models, and for investigating the evolution of host-parasite interactions.

Results

Here, we collected multi-locus sequence data from 58 rodent malaria genotypes distributed throughout 13 subspecies belonging to P. berghei, P. chabaudi, P. vinckei, and P. yoelii. We employ multi-locus methods to infer the subspecies phylogeny, and use population-genetic approaches to elucidate the selective patterns shaping the evolution of these organisms. Our results reveal a time-line for the evolution of rodent Plasmodium and suggest that all the subspecies are independently evolving lineages (i.e. species). We show that estimates of species-level polymorphism are inflated if subspecies are not explicitly recognized, and detect purifying selection at most loci.

Conclusions

Our work resolves previous inconsistencies in the phylogeny of rodent malaria parasites, provides estimates of important parameters that relate to the parasite’s natural history and provides a much-needed evolutionary context for understanding diverse biological aspects from the cross-reactivity of immune responses to parasite mating patterns.

Keywords:
Rodent malaria; Plasmodium; Phylogeny; Species delimitation; Divergence time; Molecular evolution