Open Access Highly Accessed Research article

Deep sequencing and expression of microRNAs from early honeybee (Apis mellifera) embryos reveals a role in regulating early embryonic patterning

Lisa Zondag12, Peter K Dearden1 and Megan J Wilson12*

Author Affiliations

1 Laboratory for Evolution and Development, Genetics Otago and the National Research Centre for Growth and Development, Department of Biochemistry, University of Otago, P.O. Box 56, Dunedin, 9054, New Zealand

2 Developmental Biology Laboratory, Department of Anatomy, University of Otago, P.O. Box 56, Dunedin, 9054, New Zealand

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BMC Evolutionary Biology 2012, 12:211  doi:10.1186/1471-2148-12-211

Published: 2 November 2012

Additional files

Additional file 1:

Figure S3. (A) Pseudocoloured image showing DAPI (blue) and Am-eve RNA staining in red. Am-eve RNA is detected in the cytoplasm of embryonic cells. (B) Pseudocoloured image of pri-mir-0002 RNA staining (red) overlaid with DAPI (blue) staining. Pri-mir-0002 RNA is detected in the nucleus of embryonic cells.

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Additional file 2:

Figure S4. Genome location and read count for Ame-mir-10. Genome location of Ame-mir-10 in the Hox complex, between deformed (dfd) and sex-combed reduced (Scr).

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Additional file 3:

Figure S5. Clusterdraw analysis of the upstream regions of Dme-mir-1andAme-mir-1. Cluster of twi binding sites using the clusterdraw programme [50] with background model either site at D. melanogaster (A) or A. mellifera (B). This programme has successfully identified cis-regulatory elements in Apis and Drosophila previously [22,50-52]. P values cut off on the Y-axis and position in the sequence along the x-axis.

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Additional file 4:

Figure S6. Alignment of mir-0008 and mir-0005/mir-92b pre-miRNAs. Abbreviations: Apis mellifera, Bombus impatiens, Atta cephalotes, Nasonia vitripennis, Drosophila melanogaster. Boxed are the mature miRNA sequences.

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Additional file 5:

Figure S7. Alignment of sequence reads to Apis mir-92a, mir-92b-1 and mir-0005/mir-92b pre-miRNAs.

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Additional file 6:

Table S1. Phenotype of surviving larvae (at 72 hours) following siRNA injections.

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Additional file 7:

Figure S1. Length distribution in both samples of clean small RNA reads.

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Additional file 8:

Figure S2. miRNA nucleotide bias at each position.

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