Table 3

PCR primers used to amplify CRI gene fragments. Y = C/T; R= A/G
PCR primers Primer sequence (5′-3′) Reference
AcePen_Glu-fora GCT TTT CTC CAA GGT CTA CAG CTC [33]
AcePen_Cyt1018-reva GGG TGT TCT ACT GGT TGG CTG CC [33]
Penpan_CRI-spec-180rev CRY YRT YYA CAT TAA GTG A This study
PenpanGui_CRI-spec-182rev GTC ATT ATC TAC ATT AAG TAA G This study
Penmanman_CRI-spec-185rev TTA ATA TGT CGT TGT TTA CAT G This study
Penaff_CRI-spec-186rev GTA TGT CAT TGC TTG CAT TGA GTA G This study
Acewal_CRI-spec-180rev CAT TRT CTG CAT TTA AGC GT This study
Penpan_CRI-spec-128for ACG ACT AGT TAT TAA TGC T This study
Penmanman_CRI-spec-115for CAT AAG GTA ATG CTC TAT ACA ATT This study
Penaff_CRI-spec-124for CTA TAT GAT TAA CTA TTA ATG CTC This study
Acewal_CRI-spec-130for GAT TGA CTG TCA ATG TTT GT This study
AcePen_644reva AAG GGA ACC AAC AGT GCC AAA C This study
AcePen_426reva GTT GCT GAT TTC TCG TGA GG This study

a primers with two annealing sites due to the duplication of a large fragment of the hornbills’ mt genome [33].

Sammler et al.

Sammler et al. BMC Evolutionary Biology 2012 12:203   doi:10.1186/1471-2148-12-203

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