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Open Access Research article

Mitochondrial control region I and microsatellite analyses of endangered Philippine hornbill species (Aves; Bucerotidae) detect gene flow between island populations and genetic diversity loss

Svenja Sammler1, Valerio Ketmaier12, Katja Havenstein1, Ulrike Krause1, Eberhard Curio3 and Ralph Tiedemann1*

Author Affiliations

1 Institute for Biology and Biochemistry, Unit of Evolutionary Biology/Systematic Zoology, University of Potsdam, Karl-Liebknecht-Str. 24-25, Haus 26, 14476, Potsdam, Germany

2 Department of Biology and Biotechnology “C. Darwin”, University of Rome “La Sapienza”, V.le dell’Universita’ 32, 00185, Rome, Italy

3 Ruhr-University Bochum, Faculty of Biology and Biotechnology, Conservation Biology Unit, Universitätsstr. 150, 44801, Bochum, Germany

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BMC Evolutionary Biology 2012, 12:203  doi:10.1186/1471-2148-12-203

Published: 12 October 2012

Abstract

Background

The Visayan Tarictic Hornbill (Penelopides panini) and the Walden’s Hornbill (Aceros waldeni) are two threatened hornbill species endemic to the western islands of the Visayas that constitute - between Luzon and Mindanao - the central island group of the Philippine archipelago. In order to evaluate their genetic diversity and to support efforts towards their conservation, we analyzed genetic variation in ~ 600 base pairs (bp) of the mitochondrial control region I and at 12–19 nuclear microsatellite loci. The sampling covered extant populations, still occurring only on two islands (P. panini: Panay and Negros, A. waldeni: only Panay), and it was augmented with museum specimens of extinct populations from neighboring islands. For comparison, their less endangered (= more abundant) sister taxa, the Luzon Tarictic Hornbill (P. manillae) from the Luzon and Polillo Islands and the Writhed Hornbill (A. leucocephalus) from Mindanao Island, were also included in the study. We reconstructed the population history of the two Penelopides species and assessed the genetic population structure of the remaining wild populations in all four species.

Results

Mitochondrial and nuclear data concordantly show a clear genetic separation according to the island of origin in both Penelopides species, but also unravel sporadic over-water movements between islands. We found evidence that deforestation in the last century influenced these migratory events. Both classes of markers and the comparison to museum specimens reveal a genetic diversity loss in both Visayan hornbill species, P. panini and A. waldeni, as compared to their more abundant relatives. This might have been caused by local extinction of genetically differentiated populations together with the dramatic decline in the abundance of the extant populations.

Conclusions

We demonstrated a loss in genetic diversity of P. panini and A. waldeni as compared to their sister taxa P. manillae and A. leucocephalus. Because of the low potential for gene flow and population exchange across islands, saving of the remaining birds of almost extinct local populations - be it in the wild or in captivity - is particularly important to preserve the species’ genetic potential.

Keywords:
Biogeography; Bucerotidae; Conservation genetics; Genetic diversity loss; Microsatellites; Mitochondrial control region I; Philippine archipelago; Phylogeography