Table 4

Detection of nucleotide composition at sites that are polymorphic in paired samples
Nucl. Dilution ratio
Mixture Rep. position 0:100 10:90 20:80 30:70 40:60 50:50 60:40 70:30 80:20 90:10 100:0
A x B 1 666 C C C Y Y Y Y T T T T
2 C C C Y Y Y Y T T T T
3 C C C Y Y Y Y T T T T
C x D 1 366 A A R R R R R G G G G
2 A A R R R R G G G G G
3 A A R R R R G G G G G
E x F 1 120 T T T W W W W W A A A
2 T T T W W W W W A A A
3 T T T W W W W W A A A
1 363 A A A R R R R R G G G
2 A A A R R R R R G G G
3 A A A R R R R R G G G
1 660 G G G G S S S S C C C
2 G G G G S S S S C C C
3 G G G G S S S S C C C

Dilution series were created from mixtures of bacterial 16S amplicons obtained from the following Osedax samples: (A) T1048.115 clone E6; (B) T1048.115 clone D11; (C) T1071.13 clone D4; (D) T1071.13 clone C6; (E) worm T1071.35; (F) worm T1048.110. Each mixture was replicated three times. Mixtures detected by sequencing software are marked in boldface.

Salathé and Vrijenhoek

Salathé and Vrijenhoek BMC Evolutionary Biology 2012 12:189   doi:10.1186/1471-2148-12-189

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