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Table 1
Newly defined subfamilies within glycoside hydrolase family GH5
New GH5 subfamily	Historical subfamily	Number of sequences	Present taxonomical distribution	EC number	Representative PDB structure
GH5_1	A1^b	133	Archaea Bacteria Eukaryota	3.2.1.4	2ZUN
				3.2.1.73
				3.2.1.91
GH5_2	A2^b	245	Bacteria Eukaryota	3.2.1.4	2A3H
GH5_2	A2^b	245	Bacteria Eukaryota	3.2.1.132	2A3H
GH5_4	A3 + A4^b	160	Bacteria Eukaryota	3.2.1.4	2JEQ
				3.2.1.151
				3.2.1.73
				3.2.1.8
GH5_5	A5 + A6^b,^c,^e	123	Bacteria Eukaryota	3.2.1.4	IGZJ
GH5_7	A7^d	133	Archaea Bacteria Eukaryota	3.2.1.25	IRH9
				3.2.1. 78
				2.4.1.-
GH5_8	A8^d	71	Bacteria Eukaryota	3.2.1.78	2WHL
GH5_9	A9^e	107	Eukaryota	3.2.1.58	3N9K
				3.2.1.75
				3.2.1.21
GH5_10	A10^e,f	19	Bacteria Eukaryota (Metazoa)	3.2.1.78	2C0H
GH5_11		19	Eukaryota (Plants;Fungi)	ND
GH5_12		42	Bacteria Eukaryota	3.2.1.21
GH5_12		42	Bacteria Eukaryota	3.2.1.45
GH5_13		59	Bacteria	ND
GH5_14		15	Eukaryota (Plants)	3.2.1.58
GH5_15		10	Eukaryota (Fungi)	3.2.1.75
GH5_16		10	Eukaryota (Fungi)	3.2.1.164
GH5_17		5	Bacteria	3.2.1.78
GH5_18		24	Bacteria	ND
GH5_19		23	Archaea	ND
GH5_20		17	Eukaryota (Stramenopiles)	ND
GH5_21		10	Bacteria	3.2.1.8
GH5_22		12	Bacteria Eukaryota	3.2.1.4
GH5_23		5	Eukaryota (Fungi)	3.2.1.149
GH5_23		5	Eukaryota (Fungi)	3.2.1.168
GH5_24		5	Eukaryota (Fungi)	ND
GH5_25		16	Bacteria	3.2.1.4	3MMW
GH5_25		16	Bacteria	3.2.1.78	3MMW
GH5_26		17	Bacteria	3.2.1.4
GH5_26		17	Bacteria	3.2.1.73
GH5_27		5	Eukaryota (Metazoa;Fungi)	3.2.1.123
GH5_28		8	Bacteria	3.2.1.123	2OSX
GH5_29		5	Bacteria	3.2.1.123
GH5_30		5	Eukaryota (Fungi)	ND
GH5_31		5	Eukaryota (Fungi)	3.2.1.-^g
GH5_32		5	Eukaryota (Plants; Stramenopiles)	ND
GH5_33		9	Eukaryota (Stramenopiles)	ND
GH5_34		5	Bacteria	3.2.1.-^g	2Y8K
GH5_35		5	Bacteria	ND
GH5_36		23	Bacteria	3.2.1.73	IVJZ
GH5_36		23	Bacteria	3.2.1.78	IVJZ
GH5_37		19	Bacteria	3.2.1.4	ICEN
				3.2.1.73
				3.2.1.74
GH5_38		10	Bacteria	3.2.1.-^h
GH5_39		7	Bacteria	3.2.1.4
GH5_40		8	Bacteria	ND
GH5_41		14	Bacteria	ND
GH5_42		9	Bacteria	ND
GH5_43		11	Bacteria	ND
GH5_44		24	Bacteria	ND
GH5_45		5	Bacteria	ND
GH5_46		15	Bacteria	3.2.1.-^h
GH5_47		6	Bacteria	ND
GH5_48		18	Bacteria	3.2.1.-^h
GH5_49		20	Eukaryota (Fungi)	ND
GH5_50		7	Eukaryota (Fungi)	ND
GH5_51		5	Bacteria Eukaryota (Fungi)	ND
GH5_52		6	Bacteria	3.2.1.74
GH5_53		6	Bacteria	3.2.1.74

ND Activity not determined yet.

^aExperimentally determined.

^bsee [16].

^csee [17].

^dsee [18].

^esee [19].

^fsee [20].

^gEC numbers not yet defined.

^hActive enzyme(s) present but with unclear EC number(s).

* See http://www.cazy.org webcite for most recent information.

Historical names for some subfamilies are provided, along with the taxonomical range, the characterization level and structural information from representative structures. Known enzyme activities in family GH5 are provided using the following Enzyme Classification (EC) numbers and corresponding activities: 3.2.1.4 – endo-β-1,4-glucanase or cellulase; 3.2.1.8 – endo-β-1,4-xylanase; 3.2.1.21 – β-glucosidase; 3.2.1.25 – β-mannosidase; 3.2.1.45 – β-glucocerebrosidase; 3.2.1.58 – glucan β-1,3-glucosidase; 3.2.1.73 – licheninase; 3.2.1.74 – cellodextrinase; 3.2.1.75 – glucan endo-β-1,6-glucosidase; 3.2.1.78 – mannan endo-β-1,4-mannosidase or endo-β-1,4-mannanase; 3.2.1.91 – cellulose β-1,4-cellobiosidase or cellobiohydrolase; 3.2.1.123 – endoglycoceramidase; 3.2.1.132 – chitosanase; 3.2.1.149 – β-primeverosidase; 3.2.1.151 – xyloglucan-specific endo-β-1,4-glucanase; 3.2.1.164 – endo-β-1,6-galactanase; 3.2.1.168 – hesperidin 6-O-α-L-rhamnosyl-β-glucosidase; 3.2.1.- – undefined EC numbers for β-1,3-mannanase/β-1,3-glucomannanase or for arabinoxylan-specific β-xylanase or still unclear EC numbers depending on the subfamily (see notes and text); 2.4.1.- – β-mannan transglycosidase.

Aspeborg et al. BMC Evolutionary Biology 2012 12:186 doi:10.1186/1471-2148-12-186

Table 1