Table 1

Newly defined subfamilies within glycoside hydrolase family GH5
New GH5 subfamily Historical subfamily Number of sequences Present taxonomical distribution EC number Representative PDB structure
GH5_1 A1b 133 Archaea Bacteria Eukaryota 3.2.1.4 2ZUN
3.2.1.73
3.2.1.91
GH5_2 A2b 245 Bacteria Eukaryota 3.2.1.4 2A3H
3.2.1.132
GH5_4 A3 + A4b 160 Bacteria Eukaryota 3.2.1.4 2JEQ
3.2.1.151
3.2.1.73
3.2.1.8
GH5_5 A5 + A6b,c,e 123 Bacteria Eukaryota 3.2.1.4 IGZJ
GH5_7 A7d 133 Archaea Bacteria Eukaryota 3.2.1.25 IRH9
3.2.1. 78
2.4.1.-
GH5_8 A8d 71 Bacteria Eukaryota 3.2.1.78 2WHL
GH5_9 A9e 107 Eukaryota 3.2.1.58 3N9K
3.2.1.75
3.2.1.21
GH5_10 A10e,f 19 Bacteria Eukaryota (Metazoa) 3.2.1.78 2C0H
GH5_11 19 Eukaryota (Plants;Fungi) ND
GH5_12 42 Bacteria Eukaryota 3.2.1.21
3.2.1.45
GH5_13 59 Bacteria ND
GH5_14 15 Eukaryota (Plants) 3.2.1.58
GH5_15 10 Eukaryota (Fungi) 3.2.1.75
GH5_16 10 Eukaryota (Fungi) 3.2.1.164
GH5_17 5 Bacteria 3.2.1.78
GH5_18 24 Bacteria ND
GH5_19 23 Archaea ND
GH5_20 17 Eukaryota (Stramenopiles) ND
GH5_21 10 Bacteria 3.2.1.8
GH5_22 12 Bacteria Eukaryota 3.2.1.4
GH5_23 5 Eukaryota (Fungi) 3.2.1.149
3.2.1.168
GH5_24 5 Eukaryota (Fungi) ND
GH5_25 16 Bacteria 3.2.1.4 3MMW
3.2.1.78
GH5_26 17 Bacteria 3.2.1.4
3.2.1.73
GH5_27 5 Eukaryota (Metazoa;Fungi) 3.2.1.123
GH5_28 8 Bacteria 3.2.1.123 2OSX
GH5_29 5 Bacteria 3.2.1.123
GH5_30 5 Eukaryota (Fungi) ND
GH5_31 5 Eukaryota (Fungi) 3.2.1.-g
GH5_32 5 Eukaryota (Plants; Stramenopiles) ND
GH5_33 9 Eukaryota (Stramenopiles) ND
GH5_34 5 Bacteria 3.2.1.-g 2Y8K
GH5_35 5 Bacteria ND
GH5_36 23 Bacteria 3.2.1.73 IVJZ
3.2.1.78
GH5_37 19 Bacteria 3.2.1.4 ICEN
3.2.1.73
3.2.1.74
GH5_38 10 Bacteria 3.2.1.-h
GH5_39 7 Bacteria 3.2.1.4
GH5_40 8 Bacteria ND
GH5_41 14 Bacteria ND
GH5_42 9 Bacteria ND
GH5_43 11 Bacteria ND
GH5_44 24 Bacteria ND
GH5_45 5 Bacteria ND
GH5_46 15 Bacteria 3.2.1.-h
GH5_47 6 Bacteria ND
GH5_48 18 Bacteria 3.2.1.-h
GH5_49 20 Eukaryota (Fungi) ND
GH5_50 7 Eukaryota (Fungi) ND
GH5_51 5 Bacteria Eukaryota (Fungi) ND
GH5_52 6 Bacteria 3.2.1.74
GH5_53 6 Bacteria 3.2.1.74

ND Activity not determined yet.

aExperimentally determined.

bsee [16].

csee [17].

dsee [18].

esee [19].

fsee [20].

gEC numbers not yet defined.

hActive enzyme(s) present but with unclear EC number(s).

* See http://www.cazy.org webcite for most recent information.

Historical names for some subfamilies are provided, along with the taxonomical range, the characterization level and structural information from representative structures. Known enzyme activities in family GH5 are provided using the following Enzyme Classification (EC) numbers and corresponding activities: 3.2.1.4 – endo-β-1,4-glucanase or cellulase; 3.2.1.8 – endo-β-1,4-xylanase; 3.2.1.21 – β-glucosidase; 3.2.1.25 – β-mannosidase; 3.2.1.45 – β-glucocerebrosidase; 3.2.1.58 – glucan β-1,3-glucosidase; 3.2.1.73 – licheninase; 3.2.1.74 – cellodextrinase; 3.2.1.75 – glucan endo-β-1,6-glucosidase; 3.2.1.78 – mannan endo-β-1,4-mannosidase or endo-β-1,4-mannanase; 3.2.1.91 – cellulose β-1,4-cellobiosidase or cellobiohydrolase; 3.2.1.123 – endoglycoceramidase; 3.2.1.132 – chitosanase; 3.2.1.149 – β-primeverosidase; 3.2.1.151 – xyloglucan-specific endo-β-1,4-glucanase; 3.2.1.164 – endo-β-1,6-galactanase; 3.2.1.168 – hesperidin 6-O-α-L-rhamnosyl-β-glucosidase; 3.2.1.- – undefined EC numbers for β-1,3-mannanase/β-1,3-glucomannanase or for arabinoxylan-specific β-xylanase or still unclear EC numbers depending on the subfamily (see notes and text); 2.4.1.- – β-mannan transglycosidase.

Aspeborg et al.

Aspeborg et al. BMC Evolutionary Biology 2012 12:186   doi:10.1186/1471-2148-12-186

Open Data