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Open Access Research article

Asymmetric gene introgression in two closely related Orchis species: evidence from morphometric and genetic analyses

Hans Jacquemyn1*, Rein Brys2, Olivier Honnay1 and Isabel Roldán-Ruiz3

Author Affiliations

1 Division of Plant Ecology and Systematics, Biology Department, University of Leuven, Kasteelpark Arenberg 31, Heverlee, B-3001, Belgium

2 Research Institute for Nature and Forest, Brussels, 1070, Belgium

3 Plant Sciences Unit, Institute for Agricultural and Fisheries Research ILVO, Caritasstraat 21, Melle, B-9090, Belgium

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BMC Evolutionary Biology 2012, 12:178  doi:10.1186/1471-2148-12-178

Published: 12 September 2012

Additional files

Additional file 1:

STRUCTURE analysis of 113 AFLP markers scored in 200 individuals originating from a pure Orchis militaris populations, pure O. purpurea population and a hybrid population using different values of K. Additional File 2 Figure S2a gives the most likely number of clusters. Additional File 2Figure S2b gives the results for K = 3.

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Additional file 2:

Figure S2. Genomic clines for introgression of AFLP markers from a hybrid zone between Orchis militaris and Orchis purpurea. The name of each locus is given and the P -value for the test of departure from neutrality. The solid line is the estimated cline based on individuals that lacked the dominant AFLP marker.

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Additional file 3:

Principal component plot of the first and second axis for morphometric data from putative hybrids and both parents (O. militaris and O. purpurea ).

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Additional file 4:

Floral traits measured in 200 individuals of O. militaris, O. purpurea, and their putative hybrids. Letters refer to traits listed in Materials and Methods.

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