Table 4

Comparison of RNA-seq and qPCR measurements of fold change in gene expression in selected genes
Gene SD fold change SC fold change SD accession SC accession
RNA-seq qPCR RNA-seq qPCR
Technical replicates Ubiquitin 0.9 0.1 0.6 0.1 JW512238 JW538961
hspbeta-1 #2 22.9 20.6 4.5 2.1 JW506232 JW525782
hspbeta-1 #3 24.6 13.6 4.2 4.7 JW506234 JW525781
hspbeta-1 #4 105.2 109.5 4.7 2.3 JW506233 JW525780
hspbeta-1 #5 4.9 2.8 2.1 1.8 JW519143 JW525778
hsp20 #1 38.2 47 3 7.1 JW519178 JW525783
hsp70 #2 224 35.9 7.8 2.4 JW519350 JW526654
hsp70 #3 0.7 1.1 1.8 4.5 JW506218 JW525763
hsp70 #4 0.9 0.7 1.3 1.9 JW506217 JW525759
hsp70 #5 13 8.2 3.8 4.2 JW506905 JW526653
hsp70 #15 6.1 4.4 2.6 2.8 JW502097 JW521081
hsp70 #16 2.7 1.5 1.3 1.5 JW506226 JW526652
hsp70 #17 1 0.7 1.8 2.2 JW506216 JW525758
DNAJ #1 1.1 0.5 2.1 2.5 JW503763 JW522979
DNAJ #2 1.2 1.4 1.2 2 JW504839 JW524198
DNAJ #3 1.2 0.7 1 4.2 JW503761 JW522978
DNAJ #4 1.1 0.6 0.5 0.8 JW503762 JW522980
hsp90 #1 5.8 1.3 3.3 7.5 JW506231 JW525777
Biological replicates hspbeta-1 #3 24.6 30.7 4.2 7.3 JW506234 JW525781
hsp20 #1 38.2 24.2 3 3 JW519178 JW525783
hsp70 #1 2.5 11 1.3 6 JW519142 JW525772
hsp70 #2 224 95.1 7.8 19.8 JW519350 JW526654
hsp70 #3 0.7 1 1.8 0.6 JW506218 JW525763
hsp70 #4 0.9 0.8 1.3 0.8 JW506217 JW525759
hsp70 #5 13 15 3.8 5.2 JW506905 JW526653
hsp70 #15 6.1 5.2 2.6 1 JW502097 JW521081
hsp70 #16 2.7 1.2 1.3 1.4 JW506226 JW526652
hsp70 #17 1 0.8 1.8 0.8 JW506216 JW525758
DNAJ #1 1.1 0.9 2.1 1 JW503763 JW522979
DNAJ #2 1.2 1 1.2 1 JW504839 JW524198
DNAJ #3 1.2 4.2 1 2.8 JW503761 JW522978
DNAJ #4 1.1 1.3 0.5 1.5 JW503762 JW522980

Schoville et al.

Schoville et al. BMC Evolutionary Biology 2012 12:170   doi:10.1186/1471-2148-12-170

Open Data