Table 2

Genes found in both populations with statistically significant changes in expression after thermal stress
Fold change
Contig identification SD SC Gene Ontology annotation SD Accession SC Accession
Up-regulated in both
hspbeta-1 #2 22.9 4.5 response to stress; response to heat JW506232 JW525782
hspbeta-1 #3 24.6 4.2 response to stress JW506234 JW525781
hspbeta-1 #4 105.2 4.7 response to heat JW506233 JW525780
hsp20 #1 38.17 3.0 response to stress; response to heat JW519178 JW525783
hsp70 #1 2.5 1.33 ATP binding; response to stress; auxin biosynthetic process JW519142 JW525772
hsp70 #2 224.3 7.86 mitotic cell cycle; auxin biosynthetic process; response to heat; ATP binding; response to xenobiotic stimulus; DNA replication initiation; camera- type eye morphogenesis; response to cadmium ion; negative regulation of apoptosis; nucleus JW519350 JW526654
hsp70 #5 13.0 3.8 ATP binding; response to stress; auxin biosynthetic process JW506905 JW526653
hsp70 #9 460.3 7.3 ATP binding; response to stress; auxin biosynthetic process N/A N/A
hsp90 #1 5.8 3.3 protein folding; cytoplasm; unfolded protein binding; ATP binding; response to stress JW506231 JW525777
Hypothetical protein 1.3 1.4 JW506500 JW533424
Involucrin, putative 12.6 3.8 apoptosis; protein binding JW507027 JW526798
Map kinase-interacting serine Threonine-protein kinase 1 1.7 2.3 JW507795 JW527737
Midline fasciclin 3.6 4.9 JW508028 JW527995
Polyubiquitin 2.2 2.5 viral protein processing; proteolysis; serine-type endopeptidase activity; EC 3.4.21.0 JW519561 JW535653
Transcription factor kayak 3.6 4.3 regulation of transcription, DNA-dependent; sequence- specific DNA binding; protein dimerization activity; DNA binding; transcription factor activity; nucleus JW519889 JW538428
Ubiquitin 2.4 2.4 JW519952 JW532240
Unknown 1.1 2.1 JW513666 JW533072
Unknown 7.1 3.7 JW513718 JW529892
Unknown 174.3 25.3 JW514975 JW531801
Unknown 46.2 9.4 N/A N/A
Unknown 1.8 1.4 N/A N/A
Unknown 16.4 1.9 N/A
Unknown 4.3 2 JW518153 N/A
Unknown 6.2 1.8 JW518470 JW532411
Unknown 460.3 7.3 N/A N/A
Down-regulated in both
60s ribosomal protein L14 0.79 0.58 intracellular JW518618 JW521020
ATP synthase subunit 6 0.78 0.67 oxidation reduction; mitochondrial DQ913891 DQ917374
Cellular retinoic acid- binding protein 2 0.61 0.49 lipid binding; binding; transporter activity; transport JW503430 JW522628
Cytochrome oxidase subunit 3 0.816 0.69 oxidation reduction; mitochondrial DQ913891 DQ917374
Ferritin heavy chain 1 0.48 0.49 transition metal ion binding; cellular iron ion homeostasis; oxidoreductase activity; ferric iron binding; oxidation reduction; iron ion transport JW505400 JW524830
Ferritin middle subunit 216/heavy chain polypeptide 1 0.71 0.61 cellular iron ion homeostasis; oxidoreductase activity; ferric iron binding; oxidation reduction; iron ion transport N/A JW524829
NADH dehydrogenase 4L 0.73 0.67 oxidation reduction; mitochondrial DQ913891 DQ917374
NADH dehydrogenase subunit 1 0.71 0.7 oxidation reduction; mitochondrial DQ913891 DQ917374
NADH dehydrogenase subunit 2 0.74 0.69 oxidation reduction; mitochondrial DQ913891 DQ917374
NADH dehydrogenase subunit 4 0.73 0.71 oxidation reduction; mitochondrial DQ913891 DQ917374
NADH dehydrogenase subunit 5 0.85 0.73 oxidation reduction; mitochondrial DQ913891 DQ917374
NADH dehydrogenase subunit 6 0.71 0.67 oxidation reduction; mitochondrial DQ913891 DQ917374
Nuclear protein 1/Serine threonine-protein phosphatase 0.69 0.59 binding; DNA binding JW508743 JW537408
Unknown 0.67 0.56 JW517373 JW531354
Population-specific direction of response
3,4-dihydroxy-2- butanone-4-phosphate synthase 0.42 2.81 metal ion binding; riboflavin biosynthetic process; isomerase activity; EC 4.1.99.12 JW517629 N/A
Cell wall-associated hydrolase 0.64 1.67 hydrolase activity JW503411 JW522621
Cell wall-associated hydrolase 0.53 3.39 hydrolase activity JW507653 JW527565
Cell wall-associated hydrolase 0.39 2.41 hydrolase activity JW503414 JW527566
Cell wall-associated hydrolase 0.23 1.6 hydrolase activity JW503409 JW522614
Cell wall-associated hydrolase 0.23 2.9 hydrolase activity N/A JW522624
Conserved protein 0.61 2.9 JW505232 JW524634
Conserved protein 0.57 3.0 JW504216 JW523492
Cuticle protein 248 32.9 0.46 structural constituent of cuticle JW504378 JW523655
Cuticle protein 281/502 2.3 0.3 structural constituent of cuticle JW504361 JW523649
Cuticle protein 283/409 36.6 0.24 structural constituent of cuticle JW504364 JW523634
Cuticle protein 541/577 11.0 0.27 structural constituent of cuticle JW504368 JW523638
Cuticle protein 645/704 9.3 0.42 structural constituent of cuticle JW504358 JW523633
Cuticle protein 7/414 4.6 0.32 structural constituent of cuticle JW504393 JW523650
Ferritin chloroplast expressed 2.9 0.61 iron ion homeostasis; oxidoreductase activity; iron binding JW519009 JW524836
Elegans protein 0.61 2.0 signal transducer activity; membrane; signal transduction; embryonic development ending in seed dormancy; protein binding; cellular component JW505050 JW524418
Hypothetical protein 1.5 0.65 JW519182 JW526134
Hypothetical protein 0.44 3.7 JW506539 JW526192
Hypothetical protein 0.24 4.1 JW506786 JW526524
Leucine-rich protein 0.46 4.6 JW519332 JW526187
Serine acetyltransferase 0.69 3.1 acyltransferase activity JW504201 JW537250
Unknown 4.3 0.19 N/A N/A
Unknown 2.1 0.69 JW518261 JW532321
Unknown 2.0 0.47 JW517851 JW533144
Unknown 1.7 0.69 JW518175 JW525297

N/A = contigs for which a NCBI accession was not created because their sequences were shorter than the minimum length allowed in a Transcriptome Shotgun Assembly (TSA) submission.

Schoville et al.

Schoville et al. BMC Evolutionary Biology 2012 12:170   doi:10.1186/1471-2148-12-170

Open Data