The genetic structuring of Triturus karelinii mitochondrial DNA, including that introgressed into T. macedonicus . Three groups of haplotypes are recognized: ‘Balkan ancestral’, ‘Asiatic Turkey’ and ‘Balkan derived’. Note that ‘Balkan derived’ is nested within ‘Asiatic Turkey’. The inset shows the geographical range of each group: ‘Asiatic Turkey’ in white and ‘Balkan derived’ in light grey (note the enclave); localities in which ‘Balkan ancestral’ haplotypes are found are marked with open circles (see Additional file 1 for details on haplotype distribution). The area where T. macedonicus contains T. karelinii mitochondrial DNA is outlined with a white stippled line. For each group the nucleotide diversity (π) is determined. In the phylogenetic tree, red arrows signify haplotypes that are (also) found as introgressed mitochondrial DNA in T. macedonicus. Statistically significantly supported clades (with a Bayesian posterior probability of 0.95 or higher) are denoted with an asterisk (*). In the haplotype network, pie diameter expresses haplotype frequency (Additional file 3) and bars the number of mutations along a branch if more than one. Blue pies reflect T. karelinii mitochondrial DNA found in T. karelinii and red pies T. karelinii mitochondrial DNA present in T. macedonicus. If T. karelinii haplotypes are shared between T. karelinii and T. macedonicus, frequencies are reflected by the size of the slices. The numbers in the phylogeny and haplotype network refer to T. karelinii mitochondrial DNA haplotypes as coded in Additional file 3.
Wielstra and Arntzen BMC Evolutionary Biology 2012 12:161 doi:10.1186/1471-2148-12-161