Animal, Plant, and Fungi bHLH entropies, logos, and consensus.A The bHLH normalized group entropy by position. Lower values indicate conservation, while values close to one approach unity. B The graphical representation of the amino acids at each position of the bHLH domain. Symbols representing amino acids are scaled by their bit score (a derivation of entropy) at a given position. C The 50–10 consensus sequences for Animal, Plant and Fungi. Using an alignment of bHLH domains within each Kingdom, amino acids occurring at a frequency of more than 50% at a given site are displayed. At each of these sites, additional amino acids are displayed if they are conserved in 10% or more of the sequences. The Plant and Animal consensus sequences are derived from previous work [8,13]. Boundaries between bHLH subdomains are shown at the bottom.
Sailsbery and Dean BMC Evolutionary Biology 2012 12:154 doi:10.1186/1471-2148-12-154