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Open Access Highly Accessed Research article

Population structure of Bactrocera dorsalis s.s., B. papayae and B. philippinensis (Diptera: Tephritidae) in southeast Asia: evidence for a single species hypothesis using mitochondrial DNA and wing-shape data

Mark K Schutze12*, Matthew N Krosch12, Karen F Armstrong13, Toni A Chapman14, Anna Englezou14, Anastasija Chomič3, Stephen L Cameron2, Deborah Hailstones14 and Anthony R Clarke12

Author affiliations

1 CRC for National Plant Biosecurity, LPO Box 5012, Bruce, 2617, A.C.T, Australia

2 School of Earth, Environmental and Biological Sciences, Queensland University of Technology, G.P.O. Box 2434, Brisbane, 4000, QLD, Australia

3 Bio-Protection Research Centre, Lincoln University, PO Box 84, Lincoln, 7647, Christchurch, New Zealand

4 NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Woodbridge Rd, Menangle, 2568, NSW, Australia

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Citation and License

BMC Evolutionary Biology 2012, 12:130  doi:10.1186/1471-2148-12-130

Published: 30 July 2012

Abstract

Background

Bactrocera dorsalis s.s. is a pestiferous tephritid fruit fly distributed from Pakistan to the Pacific, with the Thai/Malay peninsula its southern limit. Sister pest taxa, B. papayae and B. philippinensis, occur in the southeast Asian archipelago and the Philippines, respectively. The relationship among these species is unclear due to their high molecular and morphological similarity. This study analysed population structure of these three species within a southeast Asian biogeographical context to assess potential dispersal patterns and the validity of their current taxonomic status.

Results

Geometric morphometric results generated from 15 landmarks for wings of 169 flies revealed significant differences in wing shape between almost all sites following canonical variate analysis. For the combined data set there was a greater isolation-by-distance (IBD) effect under a ‘non-Euclidean’ scenario which used geographical distances within a biogeographical ‘Sundaland context’ (r2 = 0.772, P < 0.0001) as compared to a ‘Euclidean’ scenario for which direct geographic distances between sample sites was used (r2 = 0.217, P < 0.01). COI sequence data were obtained for 156 individuals and yielded 83 unique haplotypes with no correlation to current taxonomic designations via a minimum spanning network. BEAST analysis provided a root age and location of 540kya in northern Thailand, with migration of B. dorsalis s.l. into Malaysia 470kya and Sumatra 270kya. Two migration events into the Philippines are inferred. Sequence data revealed a weak but significant IBD effect under the ‘non-Euclidean’ scenario (r2 = 0.110, P < 0.05), with no historical migration evident between Taiwan and the Philippines. Results are consistent with those expected at the intra-specific level.

Conclusions

Bactrocera dorsalis s.s., B. papayae and B. philippinensis likely represent one species structured around the South China Sea, having migrated from northern Thailand into the southeast Asian archipelago and across into the Philippines. No migration is apparent between the Philippines and Taiwan. This information has implications for quarantine, trade and pest management.

Keywords:
Geometric morphometrics; Cytochrome c oxidase I; South China Sea biogeography; Fruit flies