Figure 5.

Subcellular localization of NmeGp1Sd and human Nme1. A) HEp-2 cells transiently transfected with pEGFPN1-Nme1 (green fluorescence) and pDsRed-NmeGp1Sd (red fluorescence). The signal is visible mainly in the cytoplasm. The overlay (yellow) shows colocalization of the human and sponge homologs. B) HEp-2 cells transiently transfected with pEGFPN1-Nme1 (green fluorescence) and pDsRed-NmeGp1Sd (red fluorescence). The signal is visible mainly in the cytoplasm. The "granum-like" structures are visible in the cytoplasm, and they are formed with both, the human and the sponge homolog. The overlay (yellow) displays the subcellular colocalization of human and sponge homologs. C) HEp-2 cells transiently transfected with pEGFPN1-Nme1. The signal is present in the cytoplasm, but also in the nucleus, although not so prominent. D) HEp-2 cells transiently transfected with pEGFPN1-NmeGp1Sd. The signal is present in the cytoplasm and in the nucleus. Bar = 10 μm.

Perina et al. BMC Evolutionary Biology 2011 11:87   doi:10.1186/1471-2148-11-87
Download authors' original image