Open Access Research article

Sponge non-metastatic Group I Nme gene/protein - structure and function is conserved from sponges to humans

Drago Perina1, Maja Herak Bosnar2, Ružica Bago2, Andreja Mikoč1, Matija Harcet1, Martina Deželjin2 and Helena Ćetković1*

Author Affiliations

1 Division of Molecular Biology, Ruđer Bošković Institute, Bijenička cesta 54, 10002 Zagreb, Croatia

2 Division of Molecular Medicine, Ruđer Bošković Institute, Bijenička cesta 54, 10002 Zagreb, Croatia

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BMC Evolutionary Biology 2011, 11:87  doi:10.1186/1471-2148-11-87

Published: 1 April 2011

Additional files

Additional file 1:

Promoter regions. The structure of NmeGp1 promoter regions from sponges S. domuncula and A. queenslandica. The most plausible putative binding sites for transcription factors identified by TFSEARCH are marked. Motifs shared with human Nme1 promoter region are boxed. Arrows denote the orientation of motifs. TSS - transcription start site.

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Additional file 2:

Maximum parsimony phylogenetic tree of Group I Nme members. Bootstrap values inferred from 1000 replicates are shown next to the branches (maximum parsimony/neighbour joining support). Accession numbers of sequences used are given in brackets after species names.

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Additional file 3:

Multiple sequence alignment of NmeGp1 proteins. Human (Nme1 and Nme2), choanoflagellate Monosiga brevicollis (NmeGp1Mb) and sponges Suberites domuncula (NmeGp1Sd), Amphimedon queenslandica (NmeGp1Aq), Sycon raphanus (NmeGp1SrA and NmeGp1SrB) and Leucetta chagosensis (NmeGp1LcA and NmeGp1LcB) proteins were aligned. Enzyme active site amino acids and amino acids necessary for the association of subunits on the hexamer are conserved and marked with *.

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