Fold expression of guppy visual opsin genes in juvenile and adult eyes. One- and two-month old juvenile (1MOJ and 2MOJ, respectively) and adult (male and female) cDNA samples are derived from left (L) and right (R) eye mRNA pools isolated from 6-12 individuals for each age or sex (see Table 1). Opsin transcript copy number was normalized to the transcript copy number (geometric mean, represented by dashed line) of three reference genes: COI, β-actin, and Myosin-HC (asterisk). Error bars (±S.E.M. of triplicate reactions) are shown for all samples. Colors represent the λmax of each opsin (based on Wavelength to RGB v.1.0 software), excluding RH1.
Laver and Taylor BMC Evolutionary Biology 2011 11:81 doi:10.1186/1471-2148-11-81