Figure 2.

Southern blot analysis of HindIII restricted genomic DNA isolated from S. singularior (lanes 1, 2, 3) and O. pardalina (lane 4). Numbers on the right indicate the size of the markers in kb. Lane 1 - S. singularior DNA isolated from twenty larvae hybridized with 5' PCR fragment of S. singularior hsp70 gene (1 - 538 bp PCR fragment of complete cDNA copy); 2 - S. singularior, DNA isolated from twenty larvae with 3'-PCR fragment of S. singularior hsp70 gene (1250 - 2340 bp PCR fragment of complete cDNA copy); 3 - S. singularior DNA isolated from five larvae with 3'-PCR fragment of S. singularior hsp70 gene (1250 - 2340 bp PCR fragment of complete cDNA copy); 4 - O. pardalina, DNA isolated from twenty larvae hybridized with PCR fragment included 298 - 1851 bp of complete O. pardalina hsp70P1 gene. Presumptive correspondence of individual hsp70 family genes (according to sequencing data) to the hybridizing bands is indicated on the left of the lanes.

Garbuz et al. BMC Evolutionary Biology 2011 11:74   doi:10.1186/1471-2148-11-74
Download authors' original image