Open Access Research article

Organization and evolution of hsp70 clusters strikingly differ in two species of Stratiomyidae (Diptera) inhabiting thermally contrasting environments

David G Garbuz1, Irina A Yushenova1, Olga G Zatsepina1, Andrey A Przhiboro2, Brian R Bettencourt3 and Michael B Evgen'ev14*

Author Affiliations

1 Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia

2 Zoological Institute, Russian Academy of Sciences, St. Petersburg 199034, Russia

3 Alnylam Pharmaceuticals, 300 Third St. Cambridge, MA 02142, USA

4 Institute of Cell Biophysics, RAS, Pushchino, Moscow region,142290, Russia

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BMC Evolutionary Biology 2011, 11:74  doi:10.1186/1471-2148-11-74

Published: 22 March 2011

Additional files

Additional file 1:

Figure S1. Alignment of hsp70S1 promoter sequences.

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Additional file 2:

Figure S2. Alignment of hsp70S2 promoter sequences.

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Figure S3. Alignment of hsp70S3 promoter sequences.

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Additional file 4:

Figure S4. Alignment of hsp70S4 promoter sequences.

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Additional file 5:

Figure S5. Alignment of hsp70S5 promoter sequences.

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Additional file 6:

Table S1. Numbers of silent and replacement fixed differences between O. pardalina hsp70 genes.

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Additional file 7:

Table S2. Results of MK (McDonald-Kreitman) tests.

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Additional file 8:

Figure S6. Alignment of Stratiomys hsp70 coding sequences.

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Additional file 9:

Figure S7. Alignment of Stratiomys hsp70 5'-UTR sequences.

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Additional file 10:

Figure S8. Alignment of hsp70S1 3'-UTR sequences.

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Additional file 11:

Figure S9. Alignment of hsp70S2 and hsp70S3 3'-UTR sequences.

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Additional file 12:

Figure S10. Alignment of hsp70S4 3'-UTR sequences.

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Additional file 13:

Figure S11. Alignment of hsp70S5 3'-UTR sequences.

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Additional file 14:

Figure S12. Deduced amino acids sequence of Hsp70S1 (S. singularior), Hsp70P1 and Hsp68 (both from O. pardalina). Comparison with paralogs from several organisms exhibiting homology to Stratiomyidae Hsp70.

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Additional file 15:

Table S3: Primers for PCR and RACEs, used in the study.

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