Identifying the interacting domains in HP1 and LHR. A) The chromo shadow domain of HP1 interacts with LHR. Full length D. melanogaster HP1 is shown with the chromo domain (CHD) and chromo-shadow domain (CSD) shaded. Fragments of HP1 that were tested for interaction with LHR in a yeast two-hybrid assay are shown below. The symbols on the right indicate non-interacting (-) and interacting (+) fragments. B) A 96 AA minimal interacting fragment (M), inferred from the LHR fragments tested, interacts with HP1. Full length LHR is shown with the BESS domain and predicted NLS (*) shaded. The locations of the three amino acids for each of the five pentamer motifs in LHR that were converted to alanine by site-directed mutagenesis and individually tested for interaction with HP1 are shown (top). None of the single-motif mutants abolished interaction with HP1 in yeast two-hybrid assays. Fragments of LHR that were tested for interaction with HP1 in a yeast two-hybrid assay are shown below.
Brideau and Barbash BMC Evolutionary Biology 2011 11:57 doi:10.1186/1471-2148-11-57