Table 1

Bacterial strains and plasmids used in this study

Bacterial strain or plasmid construct

Description

Reference


Escherichia coli XL1 Blue MRF'

Δ(mcrA) 183 Δ(mcrCB-hsdSMR-mrr) 173 endA1 supe44 thi-1 recA1 gyrA96 relA1 lac [F' proAB lacIqZΔ M15 Tn 10 (tet)]

Stratagene


pSE380

Expression vector, trc promoter, lacO operator, lacI, bla

Invitrogen


pMRK1

pla in pSE380

[19]


pMRK4

epo in pSE380

[16]


pMRK105

Pla with 5 aa from Epo: 161KGVRVNQRPG

This study


pMRK405

Epo with 5 aa from Pla: 159NQRPGKGVRV

This study


pMRK110

Pla with 10 aa from Epo: 161KGVRV→NQRPG, 262KNTI, 268VSIGASLD

This study


pMRK410

Epo with 10 aa from Pla: 159NQRPG→KGVRV, 260TIKN, 266ASLDVSIG

This study


pMRK117

Pla with 17 aa from Epo: 35ETGSNA, 88MNEQNS, 155YTSV, 161KGVRV→NQRPG, 262KN→TI, 268VSIG→ASLD

This study


pMRK417

Epo with 17 aa from Pla: 33SNAETG, 86QNSMNE, 153SVYT, 159NQRPG→KGVRV, 260TI→KN, 266ASLD→VSIG

This study


pMRK431

Epo with 31 aa from Pla: 33SNA→ETG, 86QNS→MNE, 153SV→YT, 159NQRPG→KGVRV, 260TI→KN, 266ASLD→VSIG, 26KELVHEML, 101GTDVNYATNVNH, I257T, H79N, D92E, S210D, S167N, D219G, E249D, G22S

This study


pMRK431β1

Epo with 42 aa from Pla + the signal sequence: N-terminus from Pla until aa 45; the rest similar to pMRK431

This study


pMRK4β1L1

Epo with the N-terminus from Pla until aa 45

This study


pMRK410β1L1

pMRK410 with the N-terminus from Pla until aa 45

This study


pMRK417β1L1

pMRK417 with the N-terminus from Pla until aa 45

This study


pMRK431β2

pMRK431 with β2 from Pla

This study


pMRK431β4

pMRK431 with β4 from Pla

This study


pMRK431β5

pMRK431 with β5 from Pla

This study


pMRK431β8+9

pMRK431 with β8 & β9 from Pla

This study


pMRK431β10

pMRK431 with β10 from Pla

This study


Bold text indicates the novel substitutions compared to previous hybrid. aa, amino acid

Haiko et al. BMC Evolutionary Biology 2011 11:43   doi:10.1186/1471-2148-11-43

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