Summary of the substitution analysis. Molecular models of selected Pla-Epo hybrid proteins are shown, with the substituted sites coloured black. The outer membrane (OM; the girdle area) and loops 3 and 5 are indicated. The active site amino acids are shown in a space filling representation. The table summarizes the plasminogen (Plg) activation and invasion capacities of Epo, Pla, and the Pla-Epo hybrid proteins with 10, 31, and 42 substitutions towards Pla; in total, there are 65/292 amino acid differences between mature Pla and Epo. For clarity, also the plasmid names are shown.
Haiko et al. BMC Evolutionary Biology 2011 11:43 doi:10.1186/1471-2148-11-43