Open Access Highly Accessed Research article

Deep mitochondrial divergence within a Heliconius butterfly species is not explained by cryptic speciation or endosymbiotic bacteria

Astrid G Muñoz1*, Simon W Baxter2, Mauricio Linares13 and Chris D Jiggins2

Author Affiliations

1 Instituto de Genética, Departamento de Ciencias Biologicas-Facultad de Ciencias, Universidad de los Andes, Carrera 1 No 18A - 70, P.O.Box 4976, Bogotá D.C., Colombia

2 Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, UK

3 Facultad de Ciencias Naturales y Matemáticas, Universidad del Rosario, Carrera 24 No 63C -69, Bogotá D.C., Colombia

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BMC Evolutionary Biology 2011, 11:358  doi:10.1186/1471-2148-11-358

Published: 12 December 2011

Additional files

Additional file 1:

Network of mtDNA haplotypes. The median joining network of mtDNA haplotypes is congruent with the three clades in phylogenetic analysis (see Results). The colours represent H. e. venus (white), H. e. chestertonii samples of the north (black) and south of Cauca Valley (gray).

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Additional file 2:

Matrix with FST values for mtDNA markers (lower triangle) and AFLP markers (upper triangle). Abbreviations correspond to localities. In the hybrid zone, individuals were separated by phenotype: H. e. chestertonii (CV) or H. e. venus (CV_V). Asterisk shows comparisons with significant values (P < 0.05).

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Additional file 3:

Genetic diversity from AFLP markers. Estimates of total gene diversity (HT) and within group (HS). Index of fixation (FST) was calculated grouping the populations within species (** P < 0.01).

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Additional file 4:

Principal Components Analysis of AFLP markers. The circles represent individuals of H. e. chestertonii and asterisks H. e. venus. Black circles showed individuals from south populations and gray circles those from the north.

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Additional file 5:

Sequences of species and subspecies from Genbank used in the mitochondrial analysis.

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Additional file 6:

AFLPs combination primers. Eight initial primer mixtures of selective amplifications.

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