Figure 5.

Phage restrictions by RM.Eco29k1. Triangle marks phage dilutions which are shown above. BL21(DE3)xp29k11 cells carry only gene coding for Eco29kI MTase and lack Eco29kI REase activity. BL21(DE3)xpECO29 cells carry natural pECO29 plasmid, having both MTase and REase activities of the wild type Eco29kI RMS. BL21(DE3)xp29k11+p29RM cells carry genes coding for M.Eco29kI and RM.Eco29kI enzymes on two different plasmids.