Histogram showing the distribution of expected disruptions in open reading frame in 1000 simulations of nucleotide evolution of filovirus VP35-like sequences in the bat genus Myotis. The simulations start from a center of tree (COT) ancestor with an ORF and use the best-fit model of substitution and the observed maximum likelihood tree with branch lengths as parameters. The observed number of disruptions (zero) is shown by a red bar - a value found in only 0.008 of the simulations. Note that the simulations are conservative because they do not account for selective maintenance involving indel mutations (see Figure 3).
Taylor et al. BMC Evolutionary Biology 2011 11:336 doi:10.1186/1471-2148-11-336