A phylogeny from four protein-coding genes shows that Olpidium is the only flagellated genus in a clade of terrestrial non-flagellated fungi. This maximum likelihood tree from RAxML is based on concatenated amino acid sequences of genes for the elongation factor 2, the RNA polymerase II largest and second largest subunits, and actin. Numbers on the internal nodes represent posterior probabilities and bootstrap percentages calculated by MrBayes and RAxML, respectively.
Sekimoto et al. BMC Evolutionary Biology 2011 11:331 doi:10.1186/1471-2148-11-331