Additional file 4.

Figure S3: Phylogenetic relationships of 5S rDNA for the three main variants in Pollicipes species reconstructed by means of a maximun likelihood trees. Numbers on nodes represent bootstrap values based on 100 replicates. (a) Phylogeny of A type reconstruted by K81uf + I + G model. (b) Phylogeny of E type reconstruted by SYM + I + G model. (c) Phylogeny of F and G types reconstruted by HKY + I + G model. In (b) and (c), asterisks indicate P. pollicipes sequences. Figure S4: Identified regions (not aligned) of 78 nucleotides upstream the transcriptional start site of 5S ribosomal DNA. Three conserved regions were identified. Nucleotides shaded in blue share the motif CGGCCACCGGC, those shaded in red correspond to an AT rich region that it was located about -25 bp, and the TTC sequence (shared with Bombyx mori silkworms) is shaded in green color. Figure S5: 5S ribosomal RNA predicted secondary structures of barnacles. Structures correspond to the b and c types sequences that excluding the primer-annealing regions. (a-h) Sequences used in the predicted consensus secondary structures type I Pollicipes and (i) type II Pollicipes. ((a) El04Tie09b;(b) El03Afu02b; (c) Py02Oly04b; (d) El04Afu04b, El03Afu19b, El05Afu04b, El03Afu02c, El01Tie02b, El01Tie01b; (e) El01Tie03b; (f) Py08Oly03b, Py03Oly01b; (g) El03Afu07b, El04Tie06b; (h) El03Afu16b; (i) Po06Bal01b, Po06Bal02b. Figure S6: 5S alignment. Upper line is the consensus 5S rDNA gene. White boxes represent the internal control regions (ICRs) involved in the transcription of the D. melanogaster 5S rDNA, and grey shading areas correspond to the three sequence elements that regulate transcription activity of X. laevis 5S rDNA (box A, intermediate element, and box C, from left to right). The b and c types sequences represent the second and third unit of the array.

Format: PDF Size: 309KB Download file

This file can be viewed with: Adobe Acrobat Reader

Perina et al. BMC Evolutionary Biology 2011 11:304   doi:10.1186/1471-2148-11-304