Table 2

Primers for PCR

Primer

Nucleotide sequence

Target gene

Coding amino acid sequence

Note


recA Fi

5'-GAYGCNGARCAYGCIYTNGAYCC-3'

recA

DAEHALD

ip

recA Ri

5'-ACNCCDATYTTCATNCKDATYTG-3'

recA

QIRMKIGV

ip

recA_F

5'-GATTGCATATCATTCATCTGATAACG-3'

recA

non

ep

recA_R

5'-AGTGGATTRGGATCAAGCATAGC-3'

recA

non

ep

mutY_F

5'-CCTATATGWACACCATCAAAGTTGCC-3'

mutY

non

ep

mutY_R

5'-AATACTTGGRGWAACGCCACTAATG-3'

mutY

non

ep

27F

5'-AGAGTTTGATCCTGGCTCAG-3'

16S

non

ip

1492R

5'-GGTTACCTTGTTACGACTT-3'

16S

non

ip

23S F1

5'-GGGAACTGAAACATCTAAGTACC-3'

23S

non

ip

23S R1

5'-CCCGCTTAGATGCTTTCAG-3'

23S

non

ip


ip, internal primer; ep, external primer.

16S, small subunit rRNA gene; 23S, large subunit rRNA gene.

Kuwahara et al. BMC Evolutionary Biology 2011 11:285   doi:10.1186/1471-2148-11-285

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