Additional file 4.

Phylogenetic tree of Brunfelsia spp. based on chloroplast data. Maximum likelihood phylogeny of 7 species of Brunfelsia based on analysis of chloroplast ndhF and trnLF. Numbers above branches are bootstrap support values > 50%. GenBank numbers for sequences generated here are shown in boldface. Primers used for sequence amplification are from Olmstead et al [46].

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Sanchez-Puerta et al. BMC Evolutionary Biology 2011 11:277   doi:10.1186/1471-2148-11-277